Mathew Lockett, University of North Carolina, chapel Hill
Bioanalytical Screening with a 3D Cell Culture Platform: Organ Specific Responses to Exposure
Host: Lisa Holland
Mathew Lockett, PhD
Department of Chemistry,
University of North Carolina at Chapel Hill
Tissues and organs are three-dimensional structures composed of multiple cell
types and extracellular matrices. These structures are spatially and temporally
dynamic, with cells receiving regulatory cues from both abiotic factors and intracellular
signaling molecules. Current methods of cell culture rely on monolayers of cells
grown on plasticware. While these cultures are easily prepared and analyzed, they
lack the realistic tissue architectures
and physiologically relevant microenvironments found in vivo. These simplistic models often cannot predict cellular responses to drugs, environmental toxins, or other extracellular signals. We are preparing and characterizing new 3D culture platforms and measurement tools to meet these needs, focusing on tissue-representative microenvironments with
materials and techniques readily accessible to any laboratory currently working with cells.
Department of Chemistry,
University of North Carolina at Chapel Hill
and physiologically relevant microenvironments found in vivo. These simplistic models often cannot predict cellular responses to drugs, environmental toxins, or other extracellular signals. We are preparing and characterizing new 3D culture platforms and measurement tools to meet these needs, focusing on tissue-representative microenvironments with
materials and techniques readily accessible to any laboratory currently working with cells.