Susan Olesik, Ohio State University
Alignable Nanomaterials for Rapid Biomolecule Separations
Host: Prof. Holland
Professor of Chemistry
The Ohio State University
Recent advances in analysis of proteins have increased the demand for more efficient techniques to separate intact proteins. Enhanced-fluidity liquid chromatography (EFLC) involves the addition of liquefied CO2 to conventional liquid mobile phases. The addition of liquid carbon dioxide enhances diffusivity and decreases viscosity while maintaining mixture polarity, which typically results in reduced time of analysis. Herein, EFLC will be described for the separation of proteins under both hydrophilic interaction, as well as, hydrophobic interaction liquid chromatography conditions. The impact of using these solvents on protein structure, charge state distributions (CSD) and ionization efficiencies using electrospray ionization mass spectrometry will also be described. The results will show that the addition of liquefied CO2 to the mobile phase provided an ESI-friendly and “supercharging” reagent without sacrificing chromatographic performance, which can be used to improve peptide and protein identification in large-scale application.