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Lisa Holland, Ph.D.

Professor

Holland Group Website

About

Ph.D. University of North Carolina

Research interests are in capillary electrophoresis and microfluidics applied to proteins, next-generation drugs, nanoparticles, and disease markers. 

Congratulations to Dr. Holland for her National Institutes of Health R01 and NSF awards. 

Teaching Fields

Analytical

Courses Offered

  • CHEM 310, Separations

Publications

Lu, G. and L.A. Holland, Profiling the N-Glycan Composition of IgG with Lectins and Capillary Nanogel Electrophoresis. Analytical Chemistry, Article ASAP:  DOI: 10.1021/acs.analchem.8b03725

Lu, G., C.L. Crihfield, S. Gattu, L.M. Veltri, and L.A. Holland, Capillary Electrophoresis Separations of Glycans. Chemical Reviews, 2018. 118(17): p. 7867-7885.

Holland, L.A., J.S. Carver, L.M. Veltri, R.J. Henderson, and K.D. Quedado, Enhancing Research for Undergraduates through a Nanotechnology Training Program That Utilizes Analytical and Bioanalytical Tools. Analytical and Bioanalytical Chemistry, 2018. 410(24): p. 6041-6050. 

C.L. Crihfield, G. Lu, L. Bwanali, L.M. Veltri, and L.A. Holland, Advances in Enzyme Substrate Analysis with Capillary Electrophoresis. Methods, 2018 146(15): p. 93-106. 

Davis, T.A., S.M. Patberg, L.M. Sargent, A.B. Stefaniak, and L.A. Holland, Capillary Electrophoresis Analysis of Affinity to Assess Carboxylation of Multi-Walled Carbon Nanotubes. Analytica Chimica Acta, 2018. 1027: p. 149-157.

Davis, T.A. and L.A. Holland, Peptide Probe for Multiwalled Carbon Nanotubes: Electrophoretic Assessment of the Binding Interface and Evaluation of Surface Functionalization. ACS Applied Materials & Interfaces, 2018. 10(13): p. 11311-11318.

Holland, L.A., S. Gattu, C.L. Crihfield, and L. Bwanali, Capillary Electrophoresis with Stationary Nanogel Zones of Galactosidase and Erythrina Cristagalli Lectin for the Determination of b(1-3)-Linked Galactose in Glycans. Journal of Chromatography A, 2017 1523(10): p.90-96.

Gattu, S., C.L. Crihfield, and L.A. Holland, Microscale Measurements of Michaelis–Menten Constants of Neuraminidase with Nanogel Capillary Electrophoresis for the Determination of the Sialic Acid Linkage. Analytical Chemistry, 2017. 89(1): p. 929-936.

Nyakubaya, V.T., B.C. Durney, M.C.G. Ellington, A.D. Kantes, P.A. Reed, S.E. Walter, J.R. Stueckle, and L.A. Holland, Quantification of circulating steroids in individual zebrafish using stacking to achieve nanomolar detection limits with capillary electrophoresis and UV-visible absorbance detection. Analytical and Bioanalytical Chemistry, 2015. 407(23): p. 6985-6993.

Durney, B.C., C.L. Crihfield, and L.A. Holland, Capillary electrophoresis applied to DNA: determining and harnessing sequence and structure to advance bioanalyses (2009–2014). Analytical and Bioanalytical Chemistry, 2015. 407(23): p. 6923-6938.

Durney, B.C., B.A. Bachert, H.S. Sloane, S. Lukomski, J.P. Landers, and L.A. Holland, Reversible phospholipid nanogels for deoxyribonucleic acid fragment size determinations up to 1500 base pairs and integrated sample stacking. Analytica Chimica Acta, 2015. 880: p. 136-144.

Davis, T.A., S.L. Athey, M.L. Vandevender, C.L. Crihfield, C.C. Kolanko, S. Shao, M.C. Ellington, J.K. Dicks, J.S. Carver, and L.A. Holland, Electrolysis of Water in the Secondary School Science Laboratory with Inexpensive Microfluidics. Journal of Chemical Education, 2015. 92(1): p. 116–119.

Bachert, B.A., S.J. Choi, A.K. Snyder, R.V. Rio, B.C. Durney, L.A. Holland, K. Amemiya, S.L. Welkos, J.A. Bozue, C.K. Cote, R. Berisio, and S. Lukomski, A Unique Set of the Burkholderia Collagen-Like Proteins Provides Insight into Pathogenesis, Genome Evolution and Niche Adaptation, and Infection Detection. PLoS One, 2015. 10(9): p. e0137578.

Williams, R.M., C.L. Crihfield, S. Gattu, L.A. Holland, and L.J. Sooter, In vitro selection of a single-stranded DNA molecular recognition element against atrazine. International Journal of Molecular Science, 2014. 15(8): p. 14332-47.

Tuntevski, K., B.C. Durney, A.K. Snyder, P.R. LaSala, A.P. Nayak, B.J. Green, D.H. Beezhold, R.V.M. Rio, L.A. Holland, and S. Lukomski, Aspergillus collagen-like (acl) genes: identification, sequence polymorphism and assessment for PCR-based pathogen detection. Applied and Environmental Microbiology, 2013. 79(24): p. 7882-95.

Ramakrishnan, B., A.J. Moncrief, T.A. Davis, L.A. Holland, and P.K. Qasba, Investigations on b1,4-Galactosyltransferase I using 6-sulfo-GlcNAc as acceptor sugar substrate. Glycoconjugate Journal, 2013. 30(9): p. 835-42

Durney, B.C., J.A. Lounsbury, B.L. Poe, J.P. Landers, and L.A. Holland, A Thermally Responsive Phospholipid Pseudogel: Tunable DNA Sieving with Capillary Electrophoresis. Analytical Chemistry, 2013. 85(14): p. 6617-6625.

Boulanos, S., T.A. Davis, J.A. Yang, S. Lohse, A. Alkilany, L.A. Holland, and C.J. Murphy, Nanoparticle-protein interaction: thermodynamic and kinetic study of adsorption of bovine serum albumin to gold nanoparticle surface. Langmuir, 2013. 29 (48): p. 14984-14996.

X. Wu, TJ Langan, B.C. Durney, L.A. Holland, “Thermally Responsive Phospholipid Preparations for Fluid Steering and Separation in Microfluidics”, Electrophoresis 2012, 37, 2674-81.

TJ Langan, L.A. Holland, “Capillary electrophoresis coupled to electrospray mass spectrometry through a coaxial sheath flow interface and semi-permanent phospholipid coating for the determination of oligosaccharides labeled with 1-aminopyrene-3,6,8-trisulfonic acid”, Electrophoresis 2012, 33, 607-613.

T.J. Langan, V.T. Nyakubaya, L.D. Casto, T.D. Dolan S.A. Archer-Hartmann, S.L. Yedlapalli, L.J. Sooter, L.A. Holland, “Assessment of Aptamer-Steroid Binding Using Stacking Enhanced Capillary Electrophoresis” Electrophoresis 2012, 33, 866-869.

S.A. Archer-Hartmann, C.L. Crihfield, L.A. Holland, “On-Line Enzymatic Sequencing of Glycans from Trastuzumab by Phospholipid Assisted Capillary Electrophoresis” Electrophoresis 2011, 32, 3491-3498.

S.A. Archer-Hartmann, L.A. Holland, “Self-Assembled Nanomaterials for Enhanced Chemical Separations” LC-GC North America 2011, 29(5), 384-393.

S.A. Archer-Hartmann, L.M. Sargent, D. Lowry, L.A. Holland, “Microscale Exoglycosidase Processing and Lectin Capture of Glycans with Phospholipid Assisted Capillary Electrophoresis Separations” Analytical Chemistry 2011, 83(7), 2740-2047.

L.A. Holland “Capillary Electrophoresis: Focus on Undergraduate Laboratory” Journal of Chemical Education 2011, 88(3), 254-256.

C.M. White, L.A. Holland, P Famouri, “Application of Capillary Electrophoresis to Predict Crossover Frequency of Polystyrene Particles in Dielectrophoresis”, Electrophoresis 2010, 31(15), 2664-2671.

L. Bykova, S.A. Archer-Hartmann, L.A. Holland, L. Iwanowicz, V.S. Blazer, “Steroid Determination in Fish Plasma Using Capillary Electrophoresis”, Environmental Toxicology and Chemistry 2010, 29(9), 1950-1955.

R. Luo, S.A. Archer-Hartmann, L.A. Holland, “Transformable Capillary Electrophoresis for Oligosaccharides Separation Using Phospholipid Additives”, Analytical Chemistry 2010, 82, 1228-1233.