Lisa Holland, Ph.D.

Professor

About

Ph.D. University of North Carolina

Research interests are in capillary electrophoresis and microfluidics applied to proteins, biological therapeutics, native analyses, DNA, glycans, carbohydrates, nanoparticles, and disease markers.

Congratulations to Dr. Holland for her National Institutes of Health R01 and NSF awards.

Teaching Fields

Analytical, Bioanalytical

Courses Offered

CHEM 310 Instrumentation, CHEM 511 Adv Instrumentation, CHEM 715/511 Separations, Separations in Biotechnology, CHEM 516 Bioanalyses

Publications

L.A. Holland and L.D. Casto-Boggess, Gels in Microscale Electrophoresis, Annual Reviews in Analytical Chemistry 2023, 16 (1), 161-179.

T. A. Shaffer, C.U. Herrada, A.M. Walker, L.D. Casto-Boggess, L.A. Holland*, T.R. Johnson, M.E. Jones, and Y.S. Elshamy, A Cost-Effective Microfluidic Device to Teach the Principles of Electrophoresis and Electroosmosis. Journal of Chemical Education. 2023. 100(7)L p. 2782-2788

L.D. Casto-Boggess, L.A. Holland*, P.A. Lawer-Yolar, J.A. Lucas, and J.R. Guerrette, Microscale Quantification of the Inhibition of Neuraminidase Using Capillary Nanogel Electrophoresis. Analytical Chemistry, 2022. 94(6): pp. 16151–16159.

T.R. Johnson, T.A. Shaffer, L.A. Holland*, L.M. Veltri, J.A. Lucas, Y.S. Elshamy, and P.K. Rutto, A Low-Cost and Simple Demonstration of Freezing Point Depression and Colligative Properties with Common Salts and Ice Cream. Journal of Chemical Education, 2022. 99(10): pp. 3590-3594.

Y.S. Elshamy, T.G. Strein, L.A. Holland*, C. Li, A. DeBastiani, S.J. Valentine, P. Li, J.A. Lucas, and T.A. Shaffer, Nanoflow Sheath Voltage-Free Interfacing of Capillary Electrophoresis and Mass Spectrometry for the Detection of Small Molecules. Analytical Chemistry, 2022. 94(32): pp. 11329-11336.

L.A. Holland*, Y. He, J.R. Guerrette, C.L. Crihfield, and L. Bwanali, Simple, rapid, and reproducible capillary gel electrophoresis separation and laser-induced fluorescence detection of DNA topoisomers with unmodified fused silica separation capillaries. Analytical and Bioanalytical Chemistry, 2022. 414(1): pp. 713-720.

L. Bwanali, and L.A. Holland*, Capillary Nanogel Electrophoresis for the Determination of the β1–4 Galactosyltransferase Michaelis–Menten Constant and Real-Time Addition of Galactose Residues to N-Glycans and Glycoprotein. Analytical Chemistry, 2021. 93(34): pp. 11843-11851.

L.A. Holland*, Capillary Electrophoresis in Comprehensive Glycoscience 2nd Edition, Barchi, J., Ed.; Elsevier. volume 1, 2021, pp. 290-308. ISBN: 9780128194751

Crihfield, C.L., and L.A. Holland*, Protein Sieving with Capillary Nanogel Electrophoresis, Analytical Chemistry, 2021, 93, pp. 1537–1543. https://pubs.acs.org/doi/10.1021/acs.analchem.0c03865

Veltri, L.M. and L.A. Holland*, Microfluidics for Personalized Reactions to Demonstrate Stoichiometry. Journal of Chemical Education. 2020, 97, 1035-1040. https://doi.org/10.1021/acs.jchemed.9b00544

Kristoff, CJ, Li, C. Li, P. L.A. Holland*, Vibrating Sharp-Edge Spray Ionization Interface for Capillary Electrophoresis and Mass Spectrometry, Analytical Chemistry. 2020, 92, 3006-3013 https://doi.org/10.1021/acs.analchem.9b03994.

Bwanali, L., C.L. Crihfield, E.O. Newton, V. Zeger, S. Gattu, and L.A. Holland*, Quantification of the α2-6 sialic acid linkage in branched N-glycan structures with capillary nanogel electrophoresis. Analytical Chemistry. 2020. 92(1): p. 1518-1524., https://doi.org/10.1021/acs.analchem.9b04787

Kristoff, C.J., L. Bwanali, L.M. Veltri, G.P. Gautam, P.K. Rutto, E.O. Newton, and L.A. Holland*, Challenging Bioanalyses with Capillary Electrophoresis Analytical Chemistry, 2020. 92(1): p. 49-66. https://doi.org/10.1021/acs.analchem.9b04718

Crihfield, C.L., C.J. Kristof, L.M. Veltri, C.A. Wilson, W.M. Penny, and L.A. Holland*, Semi-permanent Cationic Coating for Protein Separations. Data in Brief, 2020. 29: p. 105123, https://www.sciencedirect.com/science/article/pii/S2352340920300172

Crihfield, C.L., C.J. Kristoff, L.M. Veltri, W.M. Penny, and L.A. Holland*, Semi-permanent cationic coating for protein separations. Journal of Chromatography A, 2019. 1607(6): p. 460397. https://www.sciencedirect.com/science/article/abs/pii/S0021967319307812

Lu, G., and L.A. Holland*, Profiling the N-Glycan Composition of IgG with Lectins and Capillary Nanogel Electrophoresis. Analytical Chemistry. 2019. 91(2): p. 1375–1383.

Lu, G., C.L. Crihfield, S. Gattu, L.M. Veltri, and L.A. Holland, Capillary Electrophoresis Separations of Glycans. Chemical Reviews, 2018. 118(17): p. 7867-7885.

Holland, L.A., J.S. Carver, L.M. Veltri, R.J. Henderson, and K.D. Quedado, Enhancing Research for Undergraduates through a Nanotechnology Training Program That Utilizes Analytical and Bioanalytical Tools. Analytical and Bioanalytical Chemistry, 2018. 410(24): p. 6041-6050.

C.L. Crihfield, G. Lu, L. Bwanali, L.M. Veltri, and L.A. Holland, Advances in Enzyme Substrate Analysis with Capillary Electrophoresis. Methods, 2018 146(15): p. 93-106.

Davis, T.A., S.M. Patberg, L.M. Sargent, A.B. Stefaniak, and L.A. Holland, Capillary Electrophoresis Analysis of Affinity to Assess Carboxylation of Multi-Walled Carbon Nanotubes. Analytica Chimica Acta, 2018. 1027: p. 149-157.

Davis, T.A. and L.A. Holland, Peptide Probe for Multiwalled Carbon Nanotubes: Electrophoretic Assessment of the Binding Interface and Evaluation of Surface Functionalization. ACS Applied Materials & Interfaces, 2018. 10(13): p. 11311-11318.

Holland, L.A., S. Gattu, C.L. Crihfield, and L. Bwanali, Capillary Electrophoresis with Stationary Nanogel Zones of Galactosidase and Erythrina Cristagalli Lectin for the Determination of b(1-3)-Linked Galactose in Glycans. Journal of Chromatography A, 2017 1523(10): p.90-96.

Gattu, S., C.L. Crihfield, and L.A. Holland, Microscale Measurements of Michaelis–Menten Constants of Neuraminidase with Nanogel Capillary Electrophoresis for the Determination of the Sialic Acid Linkage. Analytical Chemistry, 2017. 89(1): p. 929-936.

Nyakubaya, V.T., B.C. Durney, M.C.G. Ellington, A.D. Kantes, P.A. Reed, S.E. Walter, J.R. Stueckle, and L.A. Holland, Quantification of circulating steroids in individual zebrafish using stacking to achieve nanomolar detection limits with capillary electrophoresis and UV-visible absorbance detection. Analytical and Bioanalytical Chemistry, 2015. 407(23): p. 6985-6993.

Durney, B.C., C.L. Crihfield, and L.A. Holland, Capillary electrophoresis applied to DNA: determining and harnessing sequence and structure to advance bioanalyses (2009–2014). Analytical and Bioanalytical Chemistry, 2015. 407(23): p. 6923-6938.

Durney, B.C., B.A. Bachert, H.S. Sloane, S. Lukomski, J.P. Landers, and L.A. Holland, Reversible phospholipid nanogels for deoxyribonucleic acid fragment size determinations up to 1500 base pairs and integrated sample stacking. Analytica Chimica Acta, 2015. 880: p. 136-144.

Davis, T.A., S.L. Athey, M.L. Vandevender, C.L. Crihfield, C.C. Kolanko, S. Shao, M.C. Ellington, J.K. Dicks, J.S. Carver, and L.A. Holland, Electrolysis of Water in the Secondary School Science Laboratory with Inexpensive Microfluidics. Journal of Chemical Education, 2015. 92(1): p. 116–119.

Bachert, B.A., S.J. Choi, A.K. Snyder, R.V. Rio, B.C. Durney, L.A. Holland, K. Amemiya, S.L. Welkos, J.A. Bozue, C.K. Cote, R. Berisio, and S. Lukomski, A Unique Set of the Burkholderia Collagen-Like Proteins Provides Insight into Pathogenesis, Genome Evolution and Niche Adaptation, and Infection Detection. PLoS One, 2015. 10(9): p. e0137578.

Williams, R.M., C.L. Crihfield, S. Gattu, L.A. Holland, and L.J. Sooter, In vitro selection of a single-stranded DNA molecular recognition element against atrazine. International Journal of Molecular Science, 2014. 15(8): p. 14332-47.

Tuntevski, K., B.C. Durney, A.K. Snyder, P.R. LaSala, A.P. Nayak, B.J. Green, D.H. Beezhold, R.V.M. Rio, L.A. Holland, and S. Lukomski, Aspergillus collagen-like (acl) genes: identification, sequence polymorphism and assessment for PCR-based pathogen detection. Applied and Environmental Microbiology, 2013. 79(24): p. 7882-95.

Ramakrishnan, B., A.J. Moncrief, T.A. Davis, L.A. Holland, and P.K. Qasba, Investigations on b1,4-Galactosyltransferase I using 6-sulfo-GlcNAc as acceptor sugar substrate. Glycoconjugate Journal, 2013. 30(9): p. 835-42

Durney, B.C., J.A. Lounsbury, B.L. Poe, J.P. Landers, and L.A. Holland, A Thermally Responsive Phospholipid Pseudogel: Tunable DNA Sieving with Capillary Electrophoresis. Analytical Chemistry, 2013. 85(14): p. 6617-6625.

Boulanos, S., T.A. Davis, J.A. Yang, S. Lohse, A. Alkilany, L.A. Holland, and C.J. Murphy, Nanoparticle-protein interaction: thermodynamic and kinetic study of adsorption of bovine serum albumin to gold nanoparticle surface. Langmuir, 2013. 29 (48): p. 14984-14996.

X. Wu, TJ Langan, B.C. Durney, L.A. Holland, “Thermally Responsive Phospholipid Preparations for Fluid Steering and Separation in Microfluidics”, Electrophoresis 2012, 37, 2674-81.

TJ Langan, L.A. Holland, “Capillary electrophoresis coupled to electrospray mass spectrometry through a coaxial sheath flow interface and semi-permanent phospholipid coating for the determination of oligosaccharides labeled with 1-aminopyrene-3,6,8-trisulfonic acid”, Electrophoresis 2012, 33, 607-613.

T.J. Langan, V.T. Nyakubaya, L.D. Casto, T.D. Dolan S.A. Archer-Hartmann, S.L. Yedlapalli, L.J. Sooter, L.A. Holland, “Assessment of Aptamer-Steroid Binding Using Stacking Enhanced Capillary Electrophoresis” Electrophoresis 2012, 33, 866-869.

S.A. Archer-Hartmann, C.L. Crihfield, L.A. Holland, “On-Line Enzymatic Sequencing of Glycans from Trastuzumab by Phospholipid Assisted Capillary Electrophoresis” Electrophoresis 2011, 32, 3491-3498.

S.A. Archer-Hartmann, L.A. Holland, “Self-Assembled Nanomaterials for Enhanced Chemical Separations” LC-GC North America 2011, 29(5), 384-393.

S.A. Archer-Hartmann, L.M. Sargent, D. Lowry, L.A. Holland, “Microscale Exoglycosidase Processing and Lectin Capture of Glycans with Phospholipid Assisted Capillary Electrophoresis Separations” Analytical Chemistry 2011, 83(7), 2740-2047.

L.A. Holland “Capillary Electrophoresis: Focus on Undergraduate Laboratory” Journal of Chemical Education 2011, 88(3), 254-256.

C.M. White, L.A. Holland, P Famouri, “Application of Capillary Electrophoresis to Predict Crossover Frequency of Polystyrene Particles in Dielectrophoresis”, Electrophoresis 2010, 31(15), 2664-2671.

L. Bykova, S.A. Archer-Hartmann, L.A. Holland, L. Iwanowicz, V.S. Blazer, “Steroid Determination in Fish Plasma Using Capillary Electrophoresis”, Environmental Toxicology and Chemistry 2010, 29(9), 1950-1955.

R. Luo, S.A. Archer-Hartmann, L.A. Holland, “Transformable Capillary Electrophoresis for Oligosaccharides Separation Using Phospholipid Additives”, Analytical Chemistry 2010, 82, 1228-1233.