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Lisa Holland, Ph.D.

Professor

Holland Group Website

About

Ph.D. University of North Carolina

Research interests are in capillary electrophoresis and microfluidics applied to proteins, next-generation drugs, nanoparticles, and disease markers. 

Congratulations to Dr. Holland for her National Institutes of Health R01 and NSF awards. 

Teaching Fields

Analytical

Courses Offered

Publications

Holland, L.A., S. Gattu, C.L. Crihfield, and L. Bwanali, Capillary Electrophoresis with Stationary Nanogel Zones of Galactosidase and Erythrina Cristagalli Lectin for the Determination of b(1-3)-Linked Galactose in Glycans. Journal of Chromatography A, in press.

Gattu, S., C.L. Crihfield, and L.A. Holland, Microscale Measurements of Michaelis–Menten Constants of Neuraminidase with Nanogel Capillary Electrophoresis for the Determination of the Sialic Acid Linkage. Analytical Chemistry, 2017. 89(1): p. 929-936.

Nyakubaya, V.T., B.C. Durney, M.C.G. Ellington, A.D. Kantes, P.A. Reed, S.E. Walter, J.R. Stueckle, and L.A. Holland, Quantification of circulating steroids in individual zebrafish using stacking to achieve nanomolar detection limits with capillary electrophoresis and UV-visible absorbance detection. Analytical and Bioanalytical Chemistry, 2015. 407(23): p. 6985-6993.

Durney, B.C., C.L. Crihfield, and L.A. Holland, Capillary electrophoresis applied to DNA: determining and harnessing sequence and structure to advance bioanalyses (2009–2014). Analytical and Bioanalytical Chemistry, 2015. 407(23): p. 6923-6938.

Durney, B.C., B.A. Bachert, H.S. Sloane, S. Lukomski, J.P. Landers, and L.A. Holland, Reversible phospholipid nanogels for deoxyribonucleic acid fragment size determinations up to 1500 base pairs and integrated sample stacking. Analytica Chimica Acta, 2015. 880: p. 136-144.

Davis, T.A., S.L. Athey, M.L. Vandevender, C.L. Crihfield, C.C. Kolanko, S. Shao, M.C. Ellington, J.K. Dicks, J.S. Carver, and L.A. Holland, Electrolysis of Water in the Secondary School Science Laboratory with Inexpensive Microfluidics. Journal of Chemical Education, 2015. 92(1): p. 116–119.

Bachert, B.A., S.J. Choi, A.K. Snyder, R.V. Rio, B.C. Durney, L.A. Holland, K. Amemiya, S.L. Welkos, J.A. Bozue, C.K. Cote, R. Berisio, and S. Lukomski, A Unique Set of the Burkholderia Collagen-Like Proteins Provides Insight into Pathogenesis, Genome Evolution and Niche Adaptation, and Infection Detection. PLoS One, 2015. 10(9): p. e0137578.

Williams, R.M., C.L. Crihfield, S. Gattu, L.A. Holland, and L.J. Sooter, In vitro selection of a single-stranded DNA molecular recognition element against atrazine. International Journal of Molecular Science, 2014. 15(8): p. 14332-47.

Tuntevski, K., B.C. Durney, A.K. Snyder, P.R. LaSala, A.P. Nayak, B.J. Green, D.H. Beezhold, R.V.M. Rio, L.A. Holland, and S. Lukomski, Aspergillus collagen-like (acl) genes: identification, sequence polymorphism and assessment for PCR-based pathogen detection. Applied and Environmental Microbiology, 2013. 79(24): p. 7882-95.

Ramakrishnan, B., A.J. Moncrief, T.A. Davis, L.A. Holland, and P.K. Qasba, Investigations on b1,4-Galactosyltransferase I using 6-sulfo-GlcNAc as acceptor sugar substrate. Glycoconjugate Journal, 2013. 30(9): p. 835-42

Durney, B.C., J.A. Lounsbury, B.L. Poe, J.P. Landers, and L.A. Holland, A Thermally Responsive Phospholipid Pseudogel: Tunable DNA Sieving with Capillary Electrophoresis. Analytical Chemistry, 2013. 85(14): p. 6617-6625.

Boulanos, S., T.A. Davis, J.A. Yang, S. Lohse, A. Alkilany, L.A. Holland, and C.J. Murphy, Nanoparticle-protein interaction: thermodynamic and kinetic study of adsorption of bovine serum albumin to gold nanoparticle surface. Langmuir, 2013. 29 (48): p. 14984-14996.

X. Wu, TJ Langan, B.C. Durney, L.A. Holland, “Thermally Responsive Phospholipid Preparations for Fluid Steering and Separation in Microfluidics”, Electrophoresis 2012, 37, 2674-81.

TJ Langan, L.A. Holland, “Capillary electrophoresis coupled to electrospray mass spectrometry through a coaxial sheath flow interface and semi-permanent phospholipid coating for the determination of oligosaccharides labeled with 1-aminopyrene-3,6,8-trisulfonic acid”, Electrophoresis 2012, 33, 607-613.

T.J. Langan, V.T. Nyakubaya, L.D. Casto, T.D. Dolan S.A. Archer-Hartmann, S.L. Yedlapalli, L.J. Sooter, L.A. Holland, “Assessment of Aptamer-Steroid Binding Using Stacking Enhanced Capillary Electrophoresis” Electrophoresis 2012, 33, 866-869.

S.A. Archer-Hartmann, C.L. Crihfield, L.A. Holland, “On-Line Enzymatic Sequencing of Glycans from Trastuzumab by Phospholipid Assisted Capillary Electrophoresis” Electrophoresis 2011, 32, 3491-3498.

S.A. Archer-Hartmann, L.A. Holland, “Self-Assembled Nanomaterials for Enhanced Chemical Separations” LC-GC North America 2011, 29(5), 384-393.

S.A. Archer-Hartmann, L.M. Sargent, D. Lowry, L.A. Holland, “Microscale Exoglycosidase Processing and Lectin Capture of Glycans with Phospholipid Assisted Capillary Electrophoresis Separations” Analytical Chemistry 2011, 83(7), 2740-2047.

L.A. Holland “Capillary Electrophoresis: Focus on Undergraduate Laboratory” Journal of Chemical Education 2011, 88(3), 254-256.

C.M. White, L.A. Holland, P Famouri, “Application of Capillary Electrophoresis to Predict Crossover Frequency of Polystyrene Particles in Dielectrophoresis”, Electrophoresis 2010, 31(15), 2664-2671.

L. Bykova, S.A. Archer-Hartmann, L.A. Holland, L. Iwanowicz, V.S. Blazer, “Steroid Determination in Fish Plasma Using Capillary Electrophoresis”, Environmental Toxicology and Chemistry 2010, 29(9), 1950-1955.

R. Luo, S.A. Archer-Hartmann, L.A. Holland, “Transformable Capillary Electrophoresis for Oligosaccharides Separation Using Phospholipid Additives”, Analytical Chemistry 2010, 82, 1228-1233.